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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 8, No 2 (2013): August 2013" : 5 Documents clear
Quality Deterioration of Boiled Salted Carp Fish (Cyprinus carprio), Processed using Different Cooking Methods, During Chilling Storage Dwi Suryaningrum, Theresia; Syamdidi, Syamdidi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.89

Abstract

Study on quality changes of boiled salted Carp  fish, processed using different cooking  methods during chilling storage has been conducted. The  study was  intended to obtain an information on  the effect of cooking method on  the shelf-life of boiled salted carp fish (Cyprinus carpio) at chilling temperatures. Fish was soaked in a 0.2% of alum solution and then marinated in condiment solution for 30 minutes. Fish were cooked for 4 h by two different methods i.e steaming and boiling,  at 98-100 oC,  and then dried  in the oven at 80-90 oC for 1 hour. Boiled salted fish  then were  stored at chilling room (2-4 oC)  and observed every 3 days for 15 days.  The quality of boiled salted  fish were analyzed  i.e proximate at the beginning and the end of storage, while moisture content, pH, TVB, total plate count, mold and sensory test were conducted  during chilling storage. The results showed that steamed boiled salted  carp  fish had higher protein content, pH and also have better product which was preferable by the panelists. Based on this result  steaming method   was recomended to be used as processing method for production  of boiled salted  carp fish from fresh water.  Sensory test found that panelists preferred steamed  product which had a good appearance, odor, taste  and texture.  However steamed product had faster increase of  moisture content, TVB, and number of  bacteria as well as the decrease of pH value and odor causing faster deterioration of product compared to boiling method. Based on microbiological  tests, the boiled salted carp fish cooked by steaming  methods  were  safe to be consumed before 6 days  and the boiling method before 9 days, since storage exceed that periods resulted in  number  of bacteria already reached the maximum number allowed  and became not suitable for human consumption.
Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta Nurrahmi Dewi Fajarningsih; Muhammad Nursid; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.25

Abstract

This research was aimed to isolate marine derived-fungi which associated with macro algae and sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml of liquid medium for 4 weeks at room temperature (27-28°C) in static conditions. The antitumor activity of the fungi extracts were tested against breast tumor cells (T47D) and cervical cancer cells (HeLa) using Thiazolyl Blue Tetrazolium Blue (MTT) assay method. A number of 21 isolates of fungi were isolated from 4 macro algae and 1 sponge samples. The identification of fungi isolate was conducted using combination of molecular approach (ITS1-5.8S-ITS4 DNA regions) and macro-micro morphological characteristics. Among those 21 marine fungi species isolated, MFGK-21 extract showed the best anti-servical tumor (HeLa) with an IC50 value of 240.1 µg/ml and MFGK-27 extract showed the best anti-breast tumor (T47D) with an IC50 value of 59.6 µg/ml. The MFGK-21 fungi isolate was identified as Penicillium steckii, while the MFGK-27 fungi isolate was identified as Aspergillus sydowii.
Identification of SGS 1609 Cellulolytic Bacteria Isolated from Sargassum spec. and Characterization of The Cellulase Produced Yusro Nuri Fawzya; Stenny Putri; Nita Noriko; Gintung Patantis
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.87

Abstract

Bacterial isolate from seaweed designated as SGS 1609 was previously found to be able to produce cellulase represented by formation of clear zone on solid medium containing carboxymethylcellulose (CMC). This research was conducted to identify the isolate and determine optimum production time as well as characterize the cellulase produced. The isolate was identified using  16s-rRNA gene analysis. Cellulase production was conducted by cultivating the isolate in the liquid medium containing CMC followed by centrifuging to get supernatant as the crude enzyme. The enzyme was then concentrated using ammonium sulfate precipitation and ultra filtration. The concentrated enzyme having higher activity produced from the concentration process was then characterized  to determine its optimum pH and temperature, heat stabilization, metal ions effect and substrate specificity. The result showed that the SGS 1609 isolate was identified as Serratia marcescens with 99%  similarity. The isolate produced cellulase optimally at 4 days incubation. Ultra filtration produced higher enzyme activity compared to NH4-sulfate precipitation. The enzyme concentrated by ultra filtration worked optimally at the  pH of 7, temperature of 50 oC, stable at the temperature of 60 oC for 240 minutes and was increased its activity by Ca2+ and Mg2+ ions. On the other hand, the enzyme was inhibited by Fe3+, Zn2+ and Na+ ions, but was not relatively affected by K+ and EDTA. The use of conventional agar producer waste  treated with 6% NaOH gave highest activity compared to other substrates.
Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity Kustiariyah Tarman; Dwi Safitri; Iriani Setyaningsih
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.88

Abstract

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.
Antibacterial Activity of Sea Cucumbers Harvested from Karimunjawa Delianis Pringgenies
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (987.262 KB) | DOI: 10.15578/squalen.v8i2.90

Abstract

This research was conducted to discover bioactive compounds in sea cucumbers collected from Karimunjawa as anti-microbial agents against several pathogenic bacteria.  Five sea cucumbers species used in this study were Teripang Gamat (Stichopus variegatus), Teripang Nanas (Stichopus chloronotus), Teripang Getah (Bohadschia mamorata), Teripang Emas (Stichopus herrmanni) and Teripang Babi (Bohadschia argus). Sea cucumbers extract were subjected to a series of anti-microbial tests using Staphylococcus aureus, Escherichia coli, Vibrio anguila, Vibrio voinivica, Bacillus subtilis, and Pseudomonas sp. Results showed that in the preliminary study, tissue extract of Bohadschia mamorata and Bohadschia argus were able to suppress the growth of all tested bacteria. Further study used these two sea cucumber extracts, and the results show that Bohadschia mamorata extract at 10 mg/ml showed the largest antibacterial (2.18 mm) towards the pathogenic bacterium Pseudomonas sp., while the smallest antibacterial zone (0.63 mm) was against the growth of Bacillus subtilis. At the concentration level of 20 mg/ml Bohadschia argus extract showed the highest antibacterial zone of 3.68 mm against Staphylococcus aureus and the lowest inhibition zone (1.75 mm) against Pseudomonas sp.

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